If stains/traces of madhiy remain on garment after sprinkling water over it, does it matter?

We know that if we sprinkle a handful of water over madhiy on a garment, it is sufficient according to the most authentic opinion. But I have a doubt. If stains or traces of madhiy still remain after sprinkling the water, do I have to sprinkle water again in that area? Or is sprinkling a handful of water once sufficient regardless of the stains or traces of madhiy that remain? And is the water that was splashed on madhiy pure?